Mini Trans-Blot ® Cell and Criterion™ Blotter These cooling systems prevent temperature fluctuations and overheating during high-intensity, extended, or native protein transfers Cooling mechanism - cooling systems consist of an ice block, a sealed ice unit, or a cooling coil that is coupled to an external cooling mechanism.Plate electrodes offer greater field strength than wire electrodes but wire electrodes may be more economical and generate less heat Electrodes - tank transfer systems use either plate or wire electrode cards.Cassettes are made of non-conductive material and designed to permit unimpeded flow of current and buffer through the gel and membrane sandwich Gel holder cassette - the gel and membrane sandwich is held together between two foam pad and filter paper sheets, and placed into the tank within a gel holder cassette.Ports on the lid allow connection points for the electrodes and are energized using an external power supply On the inside, the tank has slots for placement of the electrode cards, gel holder cassettes, and cooling element. Buffer tank and lid - the buffer tank and lid combine to fully enclose the inner chamber during electrophoresis.Tank transfer systems contain the following elements: Selection of the appropriate system is largely dictated by the gel format used for separation and the desired throughput. The tank transfer systems offered by Bio-Rad are described below, and their specifications are summarized in the table below.
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